My spouse got derivative asylum through me. However, she left home and we live appart since Jan 2011.

My friend, you should discuss with a lawyer.

Do I answer yes to n-400: have you ever applied for any kind of relief from removal, exclusion, or deportation?

Yes, you have to answer those questions as a yes.

I my 3rd dui last year,all misdemeanor's,my green card will expire 2016.Am i eligible to renew my greencard or get citizenship?

Green card you can renew. The citizenship is a different question. Have you entered some type of treatment program?

Immigration

generally no, however there are exceptions. Go see a lawyer to discuss.

Officer told me that i was too old to be enroll in school, i am 29, and therefore denied the SENTRI service!

Unfortunately, what you are asking for is a benefit and your are not entitled to benefits. Try again at another location.

Eligibility to stay in US

If all goes well, you should be able to stay and work. If not, worse case you process the H-1 at the consulate.

U visa

Hard to say without seeing the RFE.

Immigration

My friend your information is not correct. Perhaps you should go consult with a lawyer before you do anything.

I am a natural born U.S. citizen wanting to obtain permanent residency for my wife.

The average time to process a case outside the US is about one year. When your wife arrives in the US with an immigrant visa, she will be able to...

It can take 6 months or more. I have seen it take more than a year.