You must pay nonexempt employees time and one-half for all hours over 8 in a workday OR over 40 in a workweek. Don't include meal period, but include breaks. So, yes you must pay OT IF they are nonexempt employees AND they work over 8 hours in a workday.
Nonexempt employees have to be paid for all hours they work over eight in a workday, and over forty in a workweek. The terms "day" and "workweek" are defined under Labor Code section 500. It might technically be possible to not pay overtime to these employees if the workday is defined as starting and ending at 4 p.m. - but, and this is a big but, that start and end time has to continuous for all of your employees. Also, it will be obvious that the change is to avoid paying overtime.
Additionally, since your employees have two shifts of more than one hour between them, you may be liable for split shift penalties, as defined by the Industrial Wage Orders (you have one posted in your breakroom, hopefully). The split shift penalty or premium requires that you pay an employee who works a split shift (two shifts more than one hour apart) an hour of pay at the minimum wage. Now if your employees are paid well enough that their total compensation is more than the minimum wage, plus one hour (for the split shift penalty), then you don't need to worry about it.
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