Disclaimer: The materials provided below are informational and should not be relied upon as legal advice. I am not licensed in your jurisdiction.
Has the 45 days passed already? If the court order gave the business to your wife, you probably should not make any transactions on the business account. You say your court order entitles you to money and your ex-wife is refusing to pay you. You should go to the court and seek judicial remedies. You should immediately consult your own attorney to protect your legal rights.
It does not sound like you should transfer the monies from the account into your account unless you go to court first to "enforce" the $50,000.00 payment order and get court permission to do so, or have your ex forced to pay you. If you transfer the money from the account into yours, since I assume there is no court order allowing you to do that, it may make things worse than they are now.
Alejandro R. Lopez, Esq.
Law Office of Alejandro R. Lopez, P.A.
4465 Edgewater Drive,
Orlando, Fla. 32804
Ph.: (407) 649-1404
Fax: (407) 649-9890